Inosine 5'-triphosphate can dramatically increase the yield of NASBA products targeting GC-rich and intramolecular base-paired viroid RNA
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چکیده
منابع مشابه
5'-triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I.
The ATPase retinoid acid-inducible gene (RIG)-I senses viral RNA in the cytoplasm of infected cells and subsequently activates cellular antiviral defense mechanisms. RIG-I recognizes molecular structures that discriminate viral from host RNA. Here, we show that RIG-I ligands require base-paired structures in conjunction with a free 5'-triphosphate to trigger antiviral signaling. Hitherto unavai...
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Human metallothioneins (MT) are represented by a multigene family. Many of these genes are expressed differentially and in a cell type/tissue specific manner in response to a variety of inducers including heavy metals (eg. Cd, Zn, Cu) and dexamethasone (Dex) (1). One of the MT genes, MT-I (2) is expressed in a hepatoblastoma cell line (Hep-G2) in response to heavy metals but not in a lymphoblas...
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The viroid and viroid-like RNA database
The viroid and viroid-like RNA database is a compilation of all natural sequences published in journals or available from the GenBank and EMBL nucleotide sequence libraries. Several information regarding these RNA species such as the position of their self-catalytic domains and the open reading frame of the human hepatitits delta virus are provided. The database also includes a determination of...
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Background Environmental and cellular inappropriate conditions can cause damages to cells nucleotide poll. Deamination and oxidation damages interfere with cell�s vital reactions. Inosine triphosphate pyrophosphatase (ITPA), an evolutionary conserved enzyme, plays a critical role in elimination of non-canonical bases. In human genome, the ITPA gene is located on chromosome 20 short arm and tran...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/26.7.1854